Specific contacts between the bacteriophage T3, T7, and SP6 RNA polymerases and their promoters.

Sequences of three promoters for the bacteriophage SP6 RNA polymerase.

Fragments of SP6 DNA generated by cleavage with Hpa II or Taq I were cloned into the Cla I site of pBR322 and the recombinant plasmids were screened for the presence of SP6 promoter activity by transcription in vitro with purified SP6 RNA polymerase. Three plasmids having promoter activity and small inserts of SP6 DNA were characterized. Hybridization studies showed that all three cloned promot...

T7- and T3-specific RNA polymerases: characterization and mapping of the in vitro transcripts read from T3 DNA.

The late genetic region of the T3 bacteriophage genome is transcribed by a phage-specified RNA polymerase, the product of T3 gene 1. In vitro, purified T3 RNA polymerase acting with T3 DNA template synthesizes six major RNA products, with molecular weights of 5.5 X 10(6), 4.2 X 10(6), 1.7 X 10(6), 0.87 X 10(6), 0.52 X 10(6), and 0.23 X 10(6). These are designated T3 RNA species I through VI, re...

Physical mapping of hybrid bacteriophage T7/T3 RNA polymerase genes.

The late regions of the T7 and T3 bacteriophage genomes are transcribed by phage-specified RNA polymerases, the products of gene 1. Although these phage transcriptional systems share many characteristics and are obviously related, they have diverged to such an extent that neither of their respective RNA polymerases utilizes the promotor sites of the other phage at an appreciable rate. However, ...

Bacteriophage T3- and T7-directed deoxyribonucleases.

the relationship between learners critical thinking ability and their performance in the reading sections of the tofel and ielts test

the study reflected in this thesis aims at finding out relationships between critical thinking (ct), and the reading sections of tofel and ielts tests. the study tries to find any relationships between the ct ability of students and their performance on reading tests of tofel and academic ielts. however, no research has ever been conducted to investigate the relationship between ct and the read...